WNT and BMP regulate roadblocks toward cardiomyocyte differentiation: lessons learned from embryos inform human stem cell differentiation
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چکیده
منابع مشابه
Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling.
Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-catenin during the initial stage of hPSC d...
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Wnt ؉ BMP ؍ stem cell tem cell fate is determined by growth factors. But on page 309, Kléber et al. show that the right combination of growth factors can also keep cells from committing to a differentiated fate. Neural crest stem cells (NCSCs), they find, are maintained by the dual punch of Wnt and BMP. NCSCs have the potential to become many different cell types. For example, Wnt exposure of...
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Understanding molecules involved in differentiation of human pluripotent stem cells (hPSCs) into cardiomyocytes and endothelial cells is important in advancing hPSCs for cell therapy and drug testing. Here, we report that LGR5, a leucine-rich repeat-containing G-protein-coupled receptor, plays a critical role in hPSC differentiation into cardiomyocytes and endothelial cells. LGR5 expression was...
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Purpose: To investigate the in vitro differentiation process of cardiomyocyte-like cells derived from human bone marrow mesenchymal stem cells under the influence of 5-azacytidine (5-aza). Materials and Methods: After purification, human bone marrow mesenchymal stem cells were exposed to 5-aza at a concentration of 5 μmol for 5 weeks to induce cardiomyocyte differentiation. To induce differenti...
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ژورنال
عنوان ژورنال: Stem Cell Investigation
سال: 2016
ISSN: 2313-0792
DOI: 10.21037/sci.2016.07.03